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Spectroscopic genetic and structural studies on bacterial rPrPc
Reference
BS410570
Principal Investigator / Supervisor
Professor George Dodson
Co-Investigators /
Co-Supervisors
Dr Peter Bayley
,
Dr James Hope
Institution
The Francis Crick Institute
Department
Division of Protein Structure
Funding type
Research
Value (£)
166,867
Status
Completed
Type
Research Grant
Start date
10/05/1999
End date
10/05/2002
Duration
36 months
Abstract
Intact PrPc and selected constructs will be produced in large amounts using bacterial expression. The recombinant protein will be used for spectroscopic and biophysical experiments to probe the molecule's folding, stability and structure in solution and where relevant, its conversion to the PrPsc form. Mutations will be introduced on PrPc and constructs, such as the 121-231, to modify the stability of the tertiary structure. The mutated material, and the information it provides, will be used in folding and crystallisation experiments, in the PrPc to PrPsc conversion studies at IAH and to support the transgenic programme. The crystallisation programme will include experiments with the 121- 231 construct and similar constructs trimmed of mobile segments, investigations on other constructs and mutated constructs and the N-terminal octapeptide repeat. There will be co- crystallisation studies with a range of ligands, concentrating on monoclonal Fab's and metal ions. The research will be carried out jointly with the IAH and will be co- ordinated with the recently initiated programme to study the behaviour and structure of the mammalian produced intact PrPc.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Biology of Spongiform Encephalopathies - Phase 4 (BS4) [1998]
Funding Scheme
X – not Funded via a specific Funding Scheme
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