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Molecular analysis of neuropathological changes in a mouse model of scrapie

Reference	BS410534
Principal Investigator / Supervisor	Professor John Fazakerley
Co-Investigators / Co-Supervisors	Professor Alun Williams
Institution	University of Edinburgh
Department	Veterinary Pathology
Funding type	Research
Value (£)	265,070
Status	Completed
Type	Research Grant
Start date	06/01/1999
End date	06/06/2002
Duration	41 months

Abstract

The contribution of interferons, cytokines, chemokines and inflammatory cells in the neuropathogenesis of TSEs is unknown. The aim is to carry out a detailed investigation into the temporal and spatial changes in transcript levels in defined brain regions and to relate these to other aspects of the neuropathology in a murine model system. We will use the ME7 strain of scrapie in CV mice since several aspects of the neuropathology in this system are already well-characterised or are under investigation. An RNAse protection assay will be used to simultaneously measure levels of a number of transcripts including interleukins 1 alpha/beta, 2, 4, 5, 6, 7, 10, 12 and 13, interferons-(alpha, beta, gamma), GMCSF, TNFalpha/beta, TGF/beta, CD3, CD4, CD8, chemokines and metalloproteinases. The work will be in a close collaboration with the Neuropathogenesis Unit here in Edinburgh.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Biology of Spongiform Encephalopathies - Phase 4 (BS4) [1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

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