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An analysis of the loss of synaptic function in scrapie-infected CA1 pyramidal neurones of mouse hippocampus
Reference
BS204809
Principal Investigator / Supervisor
Dr Nikki Macleod
Co-Investigators /
Co-Supervisors
Dr Janet Fraser
Institution
University of Edinburgh
Department
Biomedical Sciences
Funding type
Research
Value (£)
96,710
Status
Completed
Type
Research Grant
Start date
01/09/1995
End date
01/10/1997
Duration
25 months
Abstract
We have shown that scrapie infection of hippocampal CA1 pyramidal cells profoundly disturbs synaptic function, revealed by the loss of extracellular field epsp and a greatly attenuated intracellular epsp-ipsp sequence in individual neurones. Scrapie infected neurones injected with an intracellular dye show extensive loss of dendritic spines, dendritic vacuolation and axonal sprouting. This project will focus on a detailed examination of the changes in synaptic and dendritic physiology that we have highlighted, looking in particular at synaptic function, dendritic calcium currents and the interaction of the primary excitatory input to the CA1 subfield with that from local inhibitory neurones. Dye-filled neurones will be reconstructed using light and electron microscopy to study the relationship between cell physiology, synapse loss and PrP deposition.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Biology of Spongiform Encephalopathies - Phase 2 (BS2) [1993]
Funding Scheme
X – not Funded via a specific Funding Scheme
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