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Quorum-sensing control and root-exudate mediated induction of rhizobial genes expressed in the rhizosphere
Reference
BRE13665
Principal Investigator / Supervisor
Professor J Downie
Co-Investigators /
Co-Supervisors
Institution
John Innes Centre
Department
Molecular Microbiology
Funding type
Research
Value (£)
164,588
Status
Completed
Type
Research Grant
Start date
01/10/2001
End date
30/09/2004
Duration
36 months
Abstract
Quorum-sensing mutants of Rhizobium leguminosarum appear to have altered cell surface (polysaccharide) characteristics. Adjacent to a quorum-sensing regulatory gene we identified ndvA, which is involved in exporting cyclic glucans and is induced in the rhizosphere. A separate study on protein secretion by R. leguminosarum resulted in the identification of plyB, which encodes an exopolysaccharide lyase; plyB is strongly induced in the rhizosphere and is under quorum-sensing control. Downstream of plyB is ecfP which encodes a sigma factor that clearly belongs to the extracytoplasmic class of 2sigma factors. We propose to characterise the regulation of ndvA, plyB, ecfP (and various other genes involved in surface polysaccharide) with regard to quorum- sensing control and rhizosphere expression. We will identify the rhizosphere factors that induce plyB and ndA expression and a screen for other promoters that are induced in the rhizosphere will be established by DNA sequencing and construction of mutants.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Biological Interactions in the Root Environment (BRE) [2000]
Funding Scheme
X – not Funded via a specific Funding Scheme
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