Award details

Release of organic acid to the rhizosphere: a role for anion channels

ReferenceBRE13629
Principal Investigator / Supervisor Dr Stephen Roberts
Co-Investigators /
Co-Supervisors
Mr Matthew Roberts, Professor Dale Sanders
Institution Lancaster University
DepartmentBiological Sciences
Funding typeResearch
Value (£) 209,216
StatusCompleted
TypeResearch Grant
Start date 01/06/2001
End date 31/05/2004
Duration36 months

Abstract

Organic acids secreted by roots into the rhizosphere can play key roles in phosphate and iron nutrition and tolerance to aluminium. Surprisingly a systematic study of the pathways by which organic acids cross the plasma membrane of root cells has never been made. Anion channels in Arabidopsis roots will be characterised with respect to organic acid permeation, gating, and pharmacology using a combination of patch clamp and cell marking techniques. Activity will be related to net organic acid release in plants of differing nutritional status. Anion channels will be identified by functional complementation screens of a yeast anion efflux mutant, their properties compared with those in planta. Physiological roles of cloned channels will be assessed by characterising overexpressing and knockout lines.

Summary

unavailable
Committee Closed Committee - Plant & Microbial Sciences (PMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative Biological Interactions in the Root Environment (BRE) [2000]
Funding SchemeX – not Funded via a specific Funding Scheme
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