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Use of monoclonal antibodies to quantify the relationships between competitive saprotrophic ability, rhizosphere competence and lytic enzyme production by Trichoderma spp.

Reference	BRE13618
Principal Investigator / Supervisor	Professor Nicholas Talbot
Co-Investigators / Co-Supervisors	Professor Christopher Thornton
Institution	University of Exeter
Department	Biosciences
Funding type	Research
Value (£)	206,752
Status	Completed
Type	Research Grant
Start date	01/04/2001
End date	01/04/2004
Duration	36 months

Abstract

Previous studies have shown that a relationship exists between competitive saprotrophic ability (CSA), rhizosphere competence (RC) and the production of lytic enzymes by Trichoderma spp. Monoclonal antibodies raised against the enzymes 1,3-beta-glucanase (cellulase), 1,6,beta-glucanase, chitinase and the hemicellulase alpha-galactosidase will be used to quantify the relationships between CSA, RC and lytic enzyme production by T. harzianum during antagonistic interactions with Rhizoctonia solani and Pythium ultimum in soils and composts. The effects of substrate amendments on enzyme secretion and biomass production will be quantified using MAbs specific to active biomass of the antagonist and pathogens. The work aims to define the parameters required to facilitate proliferation of beneficial antagonistic Trichoderma spp. to the detriment of pathogenic root infecting fungi.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Biological Interactions in the Root Environment (BRE) [2000]
Funding Scheme	X – not Funded via a specific Funding Scheme

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