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The regulation of nitrate uptake, compartmentation and assimilation in barley
Reference
BR301906
Principal Investigator / Supervisor
Professor Brian Gordon Forde
Co-Investigators /
Co-Supervisors
Dr P Lazzeri
,
Professor Tony Miller
Institution
Rothamsted Research
Department
Plant Biology & Crop Science
Funding type
Research
Value (£)
105,765
Status
Completed
Type
Research Grant
Start date
01/04/1995
End date
01/04/1998
Duration
36 months
Abstract
The pathway of nitrate assimilation is highly regulated, but the role of the nitrate uptake system and cellular compartmentation of NO3- in controlling flux through the pathway is poorly understood. Previous studies have been hampered by difficulties in determining the cytosolic NO3- concentration and by the lack of well-defined mutants in the NO3- uptake system. We will generate transgenic barley suspension cultures up- or down-regulated for expression of a NO3- permease and mutant cell lines deficient in nitrate reductase activity. The mechanisms responsible for regulating the cytosolic NO3- pool and for controlling the flux through the NO3- uptake system will be investigated in the parental, mutant and transgenic cell lines using a newly developed triple-barrelled NO3- -sensitive microelectrode to measure cytosolic and vacuolar NO3- concentrations and 15N-labelled NO3- to determine rates of influx and efflux across the plasma membrane.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Biochemistry of Metabolic Regulation in Plants III (BR3) [1994]
Funding Scheme
X – not Funded via a specific Funding Scheme
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