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Regulation of the sulphur flux through the sulphur-assimilatory pathway

Reference	BR301882
Principal Investigator / Supervisor	Professor Malcolm Hawkesford
Co-Investigators / Co-Supervisors	Professor David Clarkson
Institution	Rothamsted Research
Department	Plant Pathology and Microbiology
Funding type	Research
Value (£)	129,414
Status	Completed
Type	Research Grant
Start date	01/02/1995
End date	01/02/1998
Duration	36 months

Abstract

It is proposed to test the hypothesis that activities of cysteine synthase and serine acetyl transferase regulate S-flux through the S-assimilation pathway and enable co-ordination with the N-assimilation pathway, by determining metabolite pools which influence gene expression. Total extractable enzyme activities and metabolite pools will be measured, contributions of the cytosolic and chloroplast pools compared and correlated with activities of enzymes in the pathway. SATase and CSase will be isolated and compared, to determine how specific metabolite pools (o-acetyl-serine and sulphide) are controlled. Interactions of these two enzymes with each other and with substrates and metabolites will be determined. The influence of S and N nutrition on activity of the S pathway will be examined.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Biochemistry of Metabolic Regulation in Plants III (BR3) [1994]
Funding Scheme	X – not Funded via a specific Funding Scheme

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