Award details

The hexosephosphate translocator of wheat endosperm amyloplasts

Reference	BR301879
Principal Investigator / Supervisor	Professor Michael Emes
Co-Investigators / Co-Supervisors	Dr Caroline Bowsher
Institution	The University of Manchester
Department	Life Sciences
Funding type	Research
Value (£)	164,581
Status	Completed
Type	Research Grant
Start date	01/07/1994
End date	01/07/1997
Duration	36 months

Abstract

The aim of this proposal is to purify and characterise the hexosephosphate translocator (HPT) of wheat endosperm amyloplasts. The purified protein which is capable of transporting glucose 6-phosphate and glucose 1-phosphate will be incorporated into liposomes and its transport characteristics studied, particularly with respect to the stoichiometry of hexosephosphate/inorganic phosphate counter exchange. Attempts will be made to obtain full length clones of HPT from a wheat endosperm cDNA library. Site directed mutagenesis will be undertaken to determine the relationship between structure and substrate specificity of the translocator which will be expressed in yeast.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Biochemistry of Metabolic Regulation in Plants III (BR3) [1994]
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search