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Award details
Construction and application of a Campylobacter jejuni DNA microarray to investigate differential gene expression
Reference
BFP11362
Principal Investigator / Supervisor
Professor Brendan Wren
Co-Investigators /
Co-Supervisors
Institution
London Sch of Hygiene and Trop Medicine
Department
Infectious and Tropical Diseases
Funding type
Research
Value (£)
192,044
Status
Completed
Type
Research Grant
Start date
12/07/1999
End date
12/07/2002
Duration
36 months
Abstract
To cause disease Campylobacter jejuni has to adapt to a range of stress-inducing environments by the temporal and spatial regulation of its gene repertoire in response to specific environmental cues. Recently the 1,641,510 bp of the C. jejuni genome has been sequenced. It is proposed to construct an inexpensive DNA microarray of the entire C. jejuni genome for transcriptome analysis. This will be used to quantitate mRNA expression of all genes in response to given stimuli including thermal, bile and aqueous stress, and growth on chicken carcasses. Ten differentially regulated genes will be examined in more detail. These studies will lead to testable hypotheses to develop new strategies for controlling campylobacters in the food chain.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Biology of Food-Borne Pathogens (BFP) [1997]
Funding Scheme
X – not Funded via a specific Funding Scheme
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