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Inhibition of virulence gene expression by targeted redirection of RNAseP
Reference
BFP11349
Principal Investigator / Supervisor
Dr Laurence Tiley
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Veterinary Medicine
Funding type
Research
Value (£)
154,854
Status
Completed
Type
Research Grant
Start date
01/11/1999
End date
01/04/2003
Duration
41 months
Abstract
RNase P will be redirected to cleave specific virulence factor mRNAs in E. coli using short complementary RNA molecules known as External Guide Sequences (EGS). Initially the EGS's will be expressed from plasmid vectors, and the effects on gene expression assessed by direct quantitation of the RNA and protein. The consequent effects on the virulence of the organism will then be determined in tissue culture and in a rabbit model for enteropathogenic E. coli. The most effective EGS will then be delivered to E. coli using modified temperate bacteriophage. The ultimate goal of this research will be to develop temperate bacteriophage vectors to deliver EGS to EPEC/EHEC infected cattle. By targeting specific virulence factors, rather than the whole organism, it is hoped to neutralise the pathogenic potential of the organism without subjecting it to strong selective pressure.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Biology of Food-Borne Pathogens (BFP) [1997]
Funding Scheme
X – not Funded via a specific Funding Scheme
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