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An analysis of carbon metabolism and alternative respiratory pathways in Campylobacter jejuni

Reference	BFP11294
Principal Investigator / Supervisor	Professor Dave Kelly
Co-Investigators / Co-Supervisors
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	200,120
Status	Completed
Type	Research Grant
Start date	01/11/1999
End date	01/01/2003
Duration	38 months

Abstract

Despite the fact that Campylobacter jejuni is the leading cause of human gastroenteritis and is an important food-borne pathogen, remarkably little is known about the physiology and metabolism of this bacterium. In particular, the organisation of central carbon metabolism has not been systematically studied and the physiological bases for its microaerophilic nature and requirement for elevated levels of carbon dioxide for growth are unknown. Interrogation of the virtually complete genome sequence of C. jejuni 11168 suggests a hitherto unappreciated metabolic versatility, with evidence for several potential pathways for glucose catabolism, a complete citric-acid cycle and a highly branched electron transport chain. It is proposed to use a combination of enzymology and 1H/13C-Nuclear Magnetic Resonance (NMR) spectroscopy to investigate key features of C. jejuni metabolism suggested by the genome sequence: (1) the mechanism of glucose catabolism and the potential role of oxygen-labile oxidoreductases in the citric-acid cycle will be determined; (2) a kinetic analysis of pyruvate and acetyl-CoA carboxylase activities will be used to investigate the link with the CO2 requirement; and (3) evidence for the operation of alternative respiratory pathways will be obtained and their role in anaerobic growth and cellular redox balancing will be studied.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Biology of Food-Borne Pathogens (BFP) [1997]
Funding Scheme	X – not Funded via a specific Funding Scheme

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