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Characterisation of the Salmonella typhimurium transcription factor and virulence determinant SlyA

Reference	BFP11284
Principal Investigator / Supervisor	Professor J Green
Co-Investigators / Co-Supervisors
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	144,097
Status	Completed
Type	Research Grant
Start date	01/06/1999
End date	01/06/2002
Duration	36 months

Abstract

Although the transcription factor SlyA has been recognised as a virulence determinant of S. typhimurium, the genes regulated, the DNA target sequence, and the SlyA activating signal are unknown. A 2D gel electrophoretic analysis will reveal the extent of the SlyA modulon and potentially identify new pathologically significant genes. Overproduced SlyA protein will be characterised biochemically and biophysically. Specific amino acids involved in DNA binding and signal transduction will be determined. The stimulus required for SlyA activation may act directly on SlyA, or indirectly by modulating slyA expression. Both of these possibilities will be investigated by analysing SlyA* proteins for co-effectors and by seeking stationary phase regulators of slyA expression. It is anticipated that by improving our understanding of SlyA regulated gene expression new insights and targets for controlling S. typhimurium will be found.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Biology of Food-Borne Pathogens (BFP) [1997]
Funding Scheme	X – not Funded via a specific Funding Scheme

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