Award details

X-ray structure of the catalytic component of benzene dioxygenase

Reference	BCI06226
Principal Investigator / Supervisor	Professor Brian Sutton
Co-Investigators / Co-Supervisors	Professor Jeremy Richard Mason
Institution	King's College London
Department	GKT School of Biomedical Sciences
Funding type	Research
Value (£)	184,976
Status	Completed
Type	Research Grant
Start date	01/09/1996
End date	01/09/1999
Duration	36 months

Abstract

We shall determine the X-ray structure of the iron-sulphur protein component of benzene dioxygenase. Suitable crystals are available. The enzyme catalyses the cis hydroxylation of aromatic substrates, and has applications in hazardous waste detoxification, and in biotransformations for enantiospecific synthesis. This reaction has no chemical synthetic equivalent. X-ray structures of substrate complexes will be determined. The corresponding component of toluene dioxygenase will be crystallised and its structure determined by molecular replacement. Knowledge of one or both of these structures will provide a basis for mechanistic studies, and manipulation of the catalytic properties such as substrate specificity to generate novel catalysts.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Biological Chemistry Initiative (BCI) [1995]
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search