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Thionate-reducing molybdoenzymes from an enteric bacterium

ReferenceBCI05147
Principal Investigator / Supervisor Professor Benjamin Berks
Co-Investigators /
Co-Supervisors
Institution University of East Anglia
DepartmentBiological Sciences
Funding typeResearch
Value (£) 147,102
StatusCompleted
TypeResearch Grant
Start date 01/01/1996
End date 01/01/1999
Duration36 months

Abstract

Enzymes involved in the reduction of thionate compounds are currently poorly characterised at the molecular level. The molybdopterin-dependent tetrathionate reductase of Salmonella typhimurium LT2 will be studied using a combination of biochemical, genetic and spectroscopic techniques. The molybdopterin cofactor normally carries out reactions involving oxygen atom transfer. The possibility that the molybdopterin cofactor in tetrathionate reductase catalyses a physiologically novel sulphur atom transfer reaction will be investigated. Our proposal that the endothermic reduction of thiosulphate by menaquinol in S. typhimurium is driven by the protonmotive force will be tested.

Summary

unavailable
Committee Closed Committee - Biomolecular Sciences (BMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative Biological Chemistry Initiative (BCI) [1995]
Funding SchemeX – not Funded via a specific Funding Scheme
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