BBSRC Portfolio Analyser
Award details
Identification and characterisation of insecticidal protein binding sites of an epithelial membrane receptor
Reference
BCI05131
Principal Investigator / Supervisor
Professor David Ellar
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Biochemistry
Funding type
Research
Value (£)
159,998
Status
Completed
Type
Research Grant
Start date
01/11/1995
End date
01/11/1998
Duration
36 months
Abstract
With previous AFRC support we purified, cloned and sequenced the first receptor for an insecticidal protein (delta-endotoxin) from Bacillus thuringiensis. Biochemical assays and sequence comparisons show the receptor to be a member of the aminopeptidase N family found on the surface of gut epithelial cells. For a full understanding of insect specificity and of the toxin mechanism, the chemistry of the receptor sites recognised by the toxin must be determined. We have strong evidence that this binding site(s) is composed of carbohydrate structures. We are also alert to the possibility that the site may be glycopeptide. We propose to fractionate the purified receptor chemically and enzymically into peptides and glycopeptides, to identify toxin binding fragments and to analyse their structure.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Biological Chemistry Initiative (BCI) [1995]
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search