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The enzymology of the magnesium chelation and methyltransferase steps in bacteriochlorophyll biosynthesis

Reference	BCI05026
Principal Investigator / Supervisor	Krebs Professor of B Christopher Hunter
Co-Investigators / Co-Supervisors
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	156,379
Status	Completed
Type	Research Grant
Start date	02/10/1995
End date	02/10/1998
Duration	36 months

Abstract

The bch genes encoding bacteriochlorophyll biosynthesis enzymes from the photosynthetic bacterium Rhodobacter sphaeroides have been mapped, cloned and sequenced in my laboratory. BchM has been overexpressed in E. coli and has been shown to encode Mg protoporphyrin methyltransferase; we have also shown that the genes bchH, I and D, when expressed in E coli encode the magnesium chelatase. In this proposal bchH, I, D and bchM will be overexpressed in E. coli and the cognate proteins will be purified. A detailed characterisation of these important enzymes will follow, which will investigate metal and porphyrin specificity, subunit stoichiometry, substrate affinity, and the kinetics of the reactions.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Biological Chemistry Initiative (BCI) [1995]
Funding Scheme	X – not Funded via a specific Funding Scheme

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