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Action of Nasturtium xyloglucan-specific endo-(1-4)-beta-D-glucanase/xyloglucan endotransglycosylase (NXET)

Reference	BCI04934
Principal Investigator / Supervisor	Professor G Reid
Co-Investigators / Co-Supervisors	Dr Paul Bates
Institution	University of Stirling
Department	Biological and Environmental Sciences
Funding type	Research
Value (£)	103,092
Status	Completed
Type	Research Grant
Start date	01/01/1996
End date	08/02/1999
Duration	37 months

Abstract

The discovery of a new family of genes which encode enzymes catalysing the specific endo- cleavage of the (1-4)-beta-glucan chain in plant cell wall xyloglucans has stimulated intensive research on the biological significance of these enzymes. Yet neither the substrate recognition requirements which dictate xyloglucan-specificity nor the catalytic mechanisms are understood. Using Nasturtium xyloglucan-specific endo-glucanase/xyloglucan endotransglycosylase (NXET) as a model, we propose to map substrate-recognition using synthetic xyloglucan oligosaccharides as substrates. We propose also to map the active site using xyloglucan-oligosaccharide-derived specific reactive probes, and to over-express the enzyme in E. coli or Saccharomyces cerevisiae prior to base-specific gene mutation and eventual crystallisation.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Biological Chemistry Initiative (BCI) [1995]
Funding Scheme	X – not Funded via a specific Funding Scheme

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