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Synthesis of N-Glycans and glycoproteins by chemical and enzymatic in vitro methods
Reference
BCI03893
Principal Investigator / Supervisor
Professor Sabine Flitsch
Co-Investigators /
Co-Supervisors
Institution
University of Edinburgh
Department
Sch of Chemistry
Funding type
Research
Value (£)
130,525
Status
Completed
Type
Research Grant
Start date
01/11/1995
End date
01/11/1998
Duration
36 months
Abstract
The present study is concerned with the glycosyltransferases involved in the synthesis of the N-linked core saccharide of glycoproteins. The beta-1,4-mannosyl transferase from yeast has already been expressed in Escherichia coli and will be purified and fully characterised, with emphasis on lipid substrate recognition. To aid isolation and purification of further transferases, an affinity chromatography method based on acceptor substrate recognition will be developed. The lipid linked core oligosaccharides which are generated by enzymatic mannosylation will then be tested as substrates for the oligosaccharyltransferase, which transfers the core saccharides from the lipids onto peptides and proteins.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Biological Chemistry Initiative (BCI) [1995]
Funding Scheme
X – not Funded via a specific Funding Scheme
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