Award details

SEQUENCING THE GENIC PORTION OF SEEDS OF DISCOVERY ADVANCE PRE- BREEDING GERMPLASM TO UNCOVER THE GENETIC VARIATION

Reference	BBS/OS/NW/000017
Principal Investigator / Supervisor	Professor Anthony Hall
Co-Investigators / Co-Supervisors
Institution	Earlham Institute
Department	Earlham Institute Department
Funding type	Research
Value (£)	323,346
Status	Completed
Type	Research Grant
Start date	01/10/2016
End date	30/09/2019
Duration	35 months

Abstract

OBJECTIVES ? Design, build and test a genic sequence capture probe-set based on the gold standard reference of the genic portion of wheat ? Re-sequence 20 CIMMYT elite lines used as parents in CIMMYTs global breeding programs and 100 of the advanced pre-breeding derived lines ? Re-sequence 200 lines of the high biomass association panel generated from the world wheat diversity panel ? Associate performance in marginal environments with genomic regions and potentially candidate genes and SNPs ? Develop a powered by iplant/Cyverse as a community repository for phenotypic, genotypic data and bioinformatics tools developed as part of this project

Summary

Exotic wheat material provides an important source of genetic variation that can be potentially exploited in wheat breeding programs. The material can be used to identify key traits that can then be combined with elite material and evaluated. At CIMMYT 70,000 accessions of the world diversity panel have been phenotypically screen as part of the seeds of discovery project. Specifically the screens have focused on traits associated with increasing yield in marginal environments. From this material approximately 1000 advanced diverse pre-breeding lines from 200 genetics crosses between exotic material x CIMMYT- best elite lines (20) have been generated. This material is being evaluated for yield and other agronomic performance indicators at Obregon in current season 2015-16 and at seven other locations with NARS partners in Mexico and India. This is set to generate a huge amount of phenotypic data. This proposal aims to sit alongside the phenotypic analysis. It will use state-of-the-art sequencing technology to re-sequence the 20 CIMMYT elite lines used in this project and a core set of 100 lines of the advanced pre-breeding material. Through this approach we aim to identify key markers associated with both agronomic performance and yield stability. Moreover, through our re-sequencing approaches it is possible to identify not only markers but also potential candidate genes responsible for some of these traits. Identifying genes underlying traits is likely to give us an insight into the biological mechanism underpinning them. Right now this may affect how field phenotyping is done and in the long-term present targets for precision genome engineering. The project will develop markers, build bioinformatics tools and integrate their use into practical wheat-breeding programs. All of this will be made available to the international community via CIMMYT and iPlant. The project builds upon germplasm developed by Sukhwinder-Singh and utilizes exome capture technology and re-sequencing approaches developed by Anthony Hall, Neil Hall, Cristobal Uauy and Ksenia Krasileva.

Committee	Not funded via Committee
Research Topics	Crop Science, Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	Newton Fund - Initial Awards (NFIA) [2017]
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search