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Sequencing and phenotyping of samples from vaccination cohorts in ODA-recipient countries in order to deliver pilot data sets
Reference
BBS/OS/GC/000008A
Principal Investigator / Supervisor
Dr Michelle Linterman
Co-Investigators /
Co-Supervisors
Institution
Babraham Institute
Department
Babraham Institute Department
Funding type
Research
Value (£)
171,734
Status
Completed
Type
Research Grant
Start date
19/10/2016
End date
31/07/2017
Duration
9 months
Abstract
In collaboration with Professor Claudia Daubenberger, Unit Leader of Clinical Immunology at the Swiss Tropical and Public Health Institute, Basel, we will conduct a pilot study on 100 peripheral blood mononuclear cell (PBMC) samples before and after vaccination, from the Group’s ongoing vaccination studies in Tanzania. These samples are already available and have been quality-controlled. We will first test the efficacy of extracting RNA and DNA from these samples. We will then generate RNA-seq and VDJ-seq sequencing libraries which will be run on the Illumina Hiseq at the Babraham Sequencing Facility. We will perform flow cytometry analysis and use Flowsom modelling of flow cytometry data to deconvolute changes in PBMC populations. We would aim to build on this pilot in subsequent applications by applying the refined approach to additional cohorts from different ODA-recipient countries, and treated with different vaccines.
Summary
unavailable
Committee
Not funded via Committee
Research Topics
Immunology
Research Priority
X – Research Priority information not available
Research Initiative
GCRF National Institutes of Bioscience Data and Resources (GCRF NIBDR) [2016]
Funding Scheme
X – not Funded via a specific Funding Scheme
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