Award details

RevGenUK, the next generation: establishing a TILLING boutique for a UK-based reverse genetics community resource

Reference	BBS/E/T/000GP005
Principal Investigator / Supervisor	Professor Mario Caccamo
Co-Investigators / Co-Supervisors
Institution	Earlham Institute
Department	Earlham Institute Department
Funding type	Research
Value (£)	25,165
Status	Completed
Type	Institute Project
Start date	01/04/2012
End date	30/06/2015
Duration	38 months

Abstract

TILLING (Targeting Induced Local Lesions in Genomes) is a non-GM reverse genetics technology for discovering, characterising and exploiting genetic variation, based on mutation detection. Reverse genetics is a strategy whereby plants carrying mutations in target genes are identified and then phenotyped, in order to determine the function of those genes. RevGenUK is a global TILLING service previously established for Medicago truncatula and Brassica rapa. The aim of this project is to expand the species range and to implement TILLING-by-sequencing as the strategy for mutation detection. Recent advances in next generation sequencing (NGS) throughput, the ability to barcode and pool samples prior to sequencing, and increased read-lengths have made TILLING-by-sequencing a practical alternative to conventional TILLING. In addition the use of exome capture approaches allow the targeted sequencing of regions of interest within the genome. Reducing the fraction of the genome to be sequenced, enables increased multiplexing of samples, and a reduction in both cost and analysis time. Over one thousand mutagenised lines of B. rapa will undergo exome capture, resequencing and analysis to detect mutations within the captured gene regions. These mutations will be displayed within a web-accessible database enabling community access to these materials. These developments will enable RevGenUK to provide a high throughput, efficient and cost effective service at the cutting edge of reverse genetics technologies.

Summary

unavailable

Committee	Not funded via Committee
Research Topics	Crop Science, Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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