Award details

Bovine Genome Mapping

Reference	BBS/E/R/04780650
Principal Investigator / Supervisor	Dr John Williams
Co-Investigators / Co-Supervisors
Institution	The Roslin Institute
Department	The Roslin Institute Department
Funding type	Research
Value (£)	216,913
Status	Completed
Type	Institute Project
Start date	01/04/1999
End date	31/03/2003
Duration	48 months

Abstract

This project supports the development of sequencing strategies and the organisation of the genotyping and AFLP analysis of the bovine genome. The project complements existing and future EU programmes. QTL mapping programmes worldwide are yielding map locations of loci involved in a wide variety of traits, while the Roslin Bovine Resource Herd should begin to produce results from 2000/2001. We have already initiated work to verify some published QTL for milk quality and have results confirming some QTL and estimating the size of their effect. It is thus time to consider carefully how we proceed from a map location to identify the gene or genes involved. In Roslin we already have large fragment libraries to "fine map" QTL, however the limit of mapping resolution is still very poor, giving very large contigs, each containing potentially many thousands of genes. positional candidate gene cloning is currently the most successful method of identifying trait genes. That is identifying genes, that from knowledge of the physiology of trait, may be considered as candidates for having an effect and which map in the region of the QTL. These genes are then tested for mutations and the association of mutations with variations in the phenotype. The candidate genes may be from the target species, however there are relatively few genes currently mapped in cattle. Alternatively by mapping a reasonable number of genes, or ESTs in cattle it is possible to align the cattle map with hat of man, and hence use the wealth of data being produced by the human genome mapping programme.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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