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The identification of novel regulators of chondrocyte differentiation and matrix resorbtion of the chick growth plate
Reference
BBS/E/R/03780633
Principal Investigator / Supervisor
Professor Colin Farquharson
Co-Investigators /
Co-Supervisors
Institution
The Roslin Institute
Department
The Roslin Institute Department
Funding type
Research
Value (£)
161,112
Status
Completed
Type
Institute Project
Start date
01/04/1997
End date
31/05/2001
Duration
50 months
Abstract
In the growth plate, chondrocytes initially go through a proliferative phase after which they gradually differentiate into a hypertrophic phenotype. The mineralised matrix they elaborate is then invaded by cells from the underlying bone: osteoclasts, which resorb the matrix, and osteoblasts, which lay down bone. The whole process is tightly controlled by local interactions between the various populations of cells but the details of this are poorly understood. This project aims to provide a much better understanding of the molecular mechanisms that regulate chondrocyte differentiation and matrix resorption in the growth plate. It will involve the cloning and characterisation of genes that are differentially expressed in proliferating or hypertrophic chondrocytes, by applying agarose gel differential display to populations of chick chondrocytes that have been purified using Percoll density gradient centrifugation. The involvement of putative regulatory genes in chondrocyte differentiation and matrix resorption will be studied using anti-sense RNA strategies.
Summary
unavailable
Committee
Closed Committee - Animal Sciences (AS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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