Award details

Manipulation of homologous recombination

Reference	BBS/E/R/01780638
Principal Investigator / Supervisor	Dr James McWhir
Co-Investigators / Co-Supervisors
Institution	The Roslin Institute
Department	The Roslin Institute Department
Funding type	Research
Value (£)	726,562
Status	Completed
Type	Institute Project
Start date	01/04/1998
End date	31/03/2002
Duration	48 months

Abstract

Gene targeting protocols are routinely used to identify rare homologous recombination events in ES cells. New aplications of gene targeting however, (both in vivo and in vitro) would follow if the frequency of homologous recombination could be enhanced. This project seeks to develop techniques in ES and somatic cells with which to modify the expression of components of mismatch repair and double strand break repair. Two assay systems in murine ES and fibroblast cells will be used to monitor response in the frequency of gene targeting following treatment with antisense oligonucleotides. Parallel strategies will be developed for gene targetting in ovine primary fibrioblasts which were previously shown to be competent for nuclear transfer.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search