Award details

Genetic manipulation of poultry

Reference	BBS/E/R/01780637
Principal Investigator / Supervisor	Professor Helen Sang
Co-Investigators / Co-Supervisors
Institution	The Roslin Institute
Department	The Roslin Institute Department
Funding type	Research
Value (£)	619,642
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	31/03/2002
Duration	60 months

Abstract

The objectives of this programme are to improve the efficiency of our method for production of transgenic chickens and to investigate two approaches, based on our expertise, to developing alternative methods. Our method involves microinjection of gene constructs into the chicken zygote, followed by cuture of the manipulated embryos to hatch. We propose to investigate the use of a novel vector to increase the chromosomal integration frequency of the introduced constructs. We plan to use model gene constructs to determine if transgene expression is regulated in a tissue-specific and developmentally regulated manner. We will then use constructs derived from the chicken lysozome gene to investigate the parameters controlling expression of transgenes in the oviduct of laying hens. An alternative route to transgenesis is the application of nuclear transfer techniques to birds. We will investigate the application of a method for positive selection of totipotent embryonic stem cells to the chicken. This method requires the productionof stable or transiently transgenic embryos as material for selective isolation in vitro of embryo cells.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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