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Role of DNA Methylation as a determinant of successful epigenetic reprogramming by nuclear transfer

ReferenceBBS/E/R/00000848
Principal Investigator / Supervisor Professor Ian Wilmut
Co-Investigators /
Co-Supervisors
Institution The Roslin Institute
DepartmentThe Roslin Institute Department
Funding typeResearch
Value (£) 17,514
StatusCompleted
TypeInstitute Project
Start date 01/10/2000
End date 31/12/2003
Duration39 months

Abstract

Nuclear transfer using differentiated mammalian cells requires reprogramming of all lineage-specific epigentic modifications for viable offspring to result. The likely role of DNA methylation in the reprogramming process will be investigated in the sheep, where changes in DNA methylation during embryogenesis have not been studied. An understanding of the regulation and possible deregulation of DNA methylation in this species will have important implications for improving the efficiency and the normality of nuclear transfer for both indirect (through animal products) and direct (through human cell-based therapies) healthcare applications. We will examine methylation in donor cells, oocyte cytoplasm, normal and reconstructed embryos.

Summary

unavailable
Committee Closed Committee - Genes & Developmental Biology (GDB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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