Award details

Funding for stem cell sorter operator

Reference	BBS/E/R/00000687
Principal Investigator / Supervisor	Dr Thomas Burdon
Co-Investigators / Co-Supervisors
Institution	The Roslin Institute
Department	The Roslin Institute Department
Funding type	Research
Value (£)	182,945
Status	Completed
Type	Institute Project
Start date	01/04/2004
End date	31/03/2006
Duration	24 months

Abstract

Experimental manipulation of intrinsic stem cell factors can be used to regulate self-renewal and differentiation in mouse ES (mES) cells. With the development of readily propagateable hES cells we can now consider performing analogous studies in human cell lines. The aim of this project is to identify intrinsic regulatory factors (RFs) that play a functional role in controlling hES cell fate. We have recently demonstrated that transient transfection of short interfering RNAs (siRNA) can efficiently knock-down gene expression in hES cells. We will therefore apply this approach to screen for functionally relevant genes in hES cells. Expression of a select pool of candidate stem cell specific regulators identified in published data bases will be knocked down using RNAi and the consequences on stem cell self-renewal and growth will be monitored. The identification of functionally relevant hES cell regulators will increase both our understanding of stem cell biology and improve the efficiency with which we can produce medically relevant cell types.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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