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Award details
Structure and Function of Plant Assimilatory Nitrate Reductase
Reference
BBS/E/J/40004093
Principal Investigator / Supervisor
Professor David John Lowe
Co-Investigators /
Co-Supervisors
Institution
John Innes Centre
Department
John Innes Centre Department
Funding type
Research
Value (£)
60,506
Status
Completed
Type
Institute Project
Start date
01/04/1997
End date
30/09/1999
Duration
30 months
Abstract
Plant (tobacco) assimilatory nitrate reductase will be overexpressed in yeast and large quantities will be prepared for structural spectroscopic and mechanistic characterisation. This will include analysis of reductive and oxidative half reactions, steady-state and pre- steady-state kinetics and binding of substrates and inhibitors. Methodology will include UV/visible, EPR and ENDOR spectroscopies and X-ray structure determination if possible. Directed mutants will be prepared to investigate hypotheses about the function of various amino- acid residues.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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