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A systematic investigation of the conditions required for the stable transfer of T-DNA to barley and wheat using time-lapse video imaging
Reference
BBS/E/J/35003523
Principal Investigator / Supervisor
Dr David Lonsdale
Co-Investigators /
Co-Supervisors
Institution
John Innes Centre
Department
John Innes Centre Department
Funding type
Research
Value (£)
8,376
Status
Completed
Type
Institute Project
Start date
01/10/1998
End date
30/09/2001
Duration
36 months
Abstract
The proposed work will develop a series of binary and super- binary vectors based on the Mullineaux binary vectors to study the transfer of T-DNA to regenerable callus of wheat and barley. T-DNA transfer will be quantitated by time-lapse video-imaging. The affects of SAR elements, introns and reversed termination signals on enhancement of expression. reduction of position effects and gene silencing respectively will be studies in single seed derived populations using quantitative RT-PCR.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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