Award details

ANTHOcyanin production PLatform Using Suspension cultures

Reference	BBS/E/J/000CA552
Principal Investigator / Supervisor	Professor Cathie Martin
Co-Investigators / Co-Supervisors	Professor Tony Miller
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	151,580
Status	Completed
Type	Institute Project
Start date	06/05/2014
End date	31/03/2017
Duration	34 months

Abstract

We aim to develop methods for large scale production of pure anthocyanins, of varying complexity in side chain decoration, or labelled with stable isotopes from plant cells. A robust plant tissue culture system will be used to produce large amounts of anthocyanins in a process spanning the whole value-chain from synthesis in novel suspension cultures, scale-up, preparative purification, assessment of bioactivities to distribution for sales. Our unique cell lines, scale-up and purification procedures will offer reliable commercial production, for the first time. We will develop methods for enhanced production, with easier extraction, by producing cultures that excrete anthocyanins, and for production of highly decorated anthocyanins using new plant sources for cell cultures. A major component of this project will be scale up activities for culturing plant cells for specialty anthocyanin production in small industrial scale fermenters. Besides preparative extraction methods, we will develop methods for the production of purified 13C-labelled anthocyanins for research on their absorption (bioavailability) and in vivo metabolism (pre- and post-absorption) in humans and animals. A fine chemicals company will assay the quality/purity /stability and bioactivity of anthocyanins compared to those from other sources. The exponential increase in interest in dietary anthocyanins means there is already a significant market for pure anthocyanins and stable isotope labelled anthocyanins amongst medical researchers. There is significant interest in pure anthocyanins from the natural colorants sector. Pure anthocyanins will be brought to market by an SME who will commission specialty anthocyanins, develop plant-specific ANTHO-kits and expand their product catalogue. We plan to expand the EU natural colours market with new trial products through a market-led business development strategy, backed up by a quality technical data package and customer demonstrations.

Summary

unavailable

Committee	Not funded via Committee
Research Topics	Crop Science, Industrial Biotechnology, Plant Science, Synthetic Biology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search