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Engineering synthetic symbioses between plants and bacteria to deliver nitrogen to crops

Reference	BBS/E/J/000CA534
Principal Investigator / Supervisor	Professor Giles Oldroyd
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	176,861
Status	Completed
Type	Institute Project
Start date	03/02/2014
End date	31/03/2017
Duration	37 months

Abstract

Availability of nitrogen (N) is one of the principal elements limiting growth and development of crops, particularly in agricultural soils for plant production of food, feed, fibre and fuel. Nature solved the N-limitation problem via evolution of biological nitrogen fixation (BNF) in bacteria (called diazotrophs), which reduce atmospheric N2 to ammonia (NH3) that is assimilated into biological molecules. This project will develop a novel model system using the C4-grass Setaria viridis (Setaria), and its interaction with both a model endophyte (Rhizobium sp. IRBG74) and an associative bacterium, (Pseudomonas fluorescens Pf-5). Synthetic biology will be used to genetically alter Setaria and the two bacteria to ensure a lock and key interaction between plant and microbe, while maximizing nitrogen fixation by the bacteria and delivery of ammonium to the plant.

Summary

unavailable

Committee	Not funded via Committee
Research Topics	Microbiology, Plant Science, Synthetic Biology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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