Award details

High Iron Wheat - HarvestPlus Challenge Program

Reference	BBS/E/J/000CA530
Principal Investigator / Supervisor	Professor Cristobal Uauy
Co-Investigators / Co-Supervisors	Dr Janneke Balk
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	73,095
Status	Completed
Type	Institute Project
Start date	15/04/2013
End date	31/12/2015
Duration	32 months

Abstract

We will investigate two independent strategies to increase iron concentration in wheat grains. The first relates to the identification of functional vacuolar transporters in wheat. We will identify putative vacuolar iron transporters in wheat through in silico methods and determine their transport activity in yeast mutants sensitive to extracellular Fe or Zn. Validated transporters will be characterized by over-expressing them in wheat seeds using endosperm-specific promoters. In addition, we will identify EMS mutants for those transporters found to be expressed in leaves. We will also examine the regulatory network modulated by GPC1 (Grain Protein Content 1). This gene has been shown to increase N, Zn and Fe remobilization from wheat flag leaves to grain, yet the downstream targets remain unknown. We will use ChIP-Seq to identify these target genes and perform an initial validation using qPCR.

Summary

unavailable

Committee	Not funded via Committee
Research Topics	Crop Science, Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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