Award details

Engineering the Sym pathway of cereals for recognition of nitrogen fixing bacteria

Reference	BBS/E/J/000CA491
Principal Investigator / Supervisor	Professor Giles Oldroyd
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	1,244,075
Status	Current
Type	Institute Project
Start date	18/06/2012
End date	30/06/2017
Duration	60 months

Abstract

We will initiate the first steps towards the transfer of biological nitrogen fixation to cereals, through engineering nodulation signalling. This represents a complex problem. However, the knowledge gained in legumes reveals that much of the machinery necessary for nodulation signalling are present in cereals. In this proposal we will attempt to engineer the symbiosis (Sym) signalling pathway of cereals to allow recognition of rhizobial bacteria and initiation of nodule organogenesis. The tools generated in legumes, in particular the gain of function mutations, allows the isolated study of the component parts of these signalling processes: Nod factor activation of calcium oscillations, calcium activation of gene expression and cytokinin induction of cell division. This work will allow us to assess what the minimal requirements are for Nod factor induction of the Sym pathway and whether Sym pathway engineering is sufficient for nodule organogenesis. The objectives of the proposal are: 1.Define the innate capability of cereals to perceive lipo-chito oligosaccharides. 2.Engineer cereals for the perception of Nod factor. 3.Engineer cereals for CCaMK induced nodulation gene expression. 4.Engineer cereals for cytokinin activation of nodule organogenesis.

Summary

unavailable

Committee	Not funded via Committee
Research Topics	Crop Science, Plant Science, Synthetic Biology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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