Award details

Generation of Alkaloid Diversity Using Biocatalysts

Reference	BBS/E/J/000CA489
Principal Investigator / Supervisor	Professor Sarah O'Connor
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	45,888
Status	Completed
Type	Institute Project
Start date	03/12/2012
End date	30/11/2015
Duration	35 months

Abstract

The enzymes responsible for the biosynthesis of natural products represent a phenomenal starting point for mechanistic and engineering studies. In this proposal we use enzyme engineering as both a tool to understand mechanism and as a means to generate enzymes with new catalytic activities. General Objectives - Collectively, the experiments described in this proposal will enable the generation of a broad base of biocatalysts that will catalyse the stereoselective production of a variety of beta-carbolines, a chemical scaffold widely used in pharmaceuticals. The work will also provide a mechanistic framework to understand the mode of stereoselectivity in other enzymes. We focus on the Pictet-Spenglerase from indole alkaloid biosynthesis, strictosidine synthase, as well as several halogenases that catalyse chlorination of indoles.

Summary

unavailable

Committee	Not funded via Committee
Research Topics	Industrial Biotechnology, Structural Biology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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