Award details

Synthesis and Evaluation of Prodrug Iminosugars

Reference	BBS/E/J/000CA469
Principal Investigator / Supervisor	Dr Rob Field
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	49,070
Status	Completed
Type	Institute Project
Start date	01/01/2012
End date	31/12/2013
Duration	24 months

Abstract

In order to dissect the molecular basis of cereal starch metabolism, this project will take a novel chemical genetics approach through the employment of small molecule inhibitors to directly manipulate carbohydrate-active enzyme activities in germinating barley seed. Based on recent results from Prof Field’s group, this project concerns the development of a series of enzymatically hydrolysable alpha-1,4-glycosylated iminosugars with tissue-specific activity in germinating barley seeds, acting as novel pro-drug inhibitors of alpha-glucosidase and control enzymatically noncleavable analogues. This will involve the exploration of new chemical and enzymatic routes for the synthesis of the target molecules, in vitro enzyme inhibition studies through activity assays against barley glycosidases, analysis of enzyme-inhibitor complexes by X-ray crystallography, and in vivo analysis on germinating barley seed (phenotype screening as well as metabolite and glycoprotein N-glycan analysis).

Summary

unavailable

Committee	Not funded via Committee
Research Topics	Crop Science, Plant Science, Structural Biology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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