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Genetic modification of barley for improved drought tolerance

Reference	BBS/E/J/000CA410
Principal Investigator / Supervisor	Professor Wendy Harwood
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	51,996
Status	Completed
Type	Institute Project
Start date	01/01/2010
End date	31/12/2011
Duration	24 months

Abstract

This collaborative project with the University of Jordan will examine possible strategies for the improvement of drought tolerance in barley varieties currently of agronomic importance in Jordan. The project has two main objectives: 1. To develop efficient regeneration systems, either from immature embryos or from mature embryo-derived callus, for the cultivars Rum and/or Mutah. Once good regeneration is established, to develop Agrobacterium-mediated transformation methods for these cultivars. 2. To test specific promoters that may be of value in a strategy to engineer drought tolerance and to test specific candidate genes, firstly in the model variety Golden Promise and later in Rum and/or Mutah. The experimental approach will be to firstly test our established regeneration and transformation protocol, used routinely for Golden Promise, with the Jordanian varieties. The culture conditions and transformation conditions will then be adapted as necessary to develop a transformation system for the Jordanian varieties. The candidate genes and promoters will be introduced into Golden Promise and the resulting transgenic plants will be assayed using a range of molecular and phenotyping techniques. If a transformation system can be developed for either Rum and /or Mutah, then the best candidate genes will also be transferred to these varieties.

Summary

unavailable

Committee	Not funded via Committee
Research Topics	Crop Science, Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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