Award details

From rice to orphan crops: robust, high throughput genetic markers for all the grasses

Reference	BBS/E/J/000CA316
Principal Investigator / Supervisor	Professor Simon Griffiths
Co-Investigators / Co-Supervisors	Professor John Snape
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	3,210
Status	Completed
Type	Institute Project
Start date	01/06/2007
End date	30/09/2008
Duration	16 months

Abstract

SSCPs are a robust and flexible marker system for the grasses. In this study nine hundred and forty FAM labelled COS markers will be developed for resolution as SSCPs on an ABI 3730 capillary electrophoresis machine. To demonstrate cross species use 140 of the markers will be tested across ten grass species. All 940 will be screened against the wheat varieties Avalon, Cadenza, Synthetic and Opata. Of the polymorphic markers, the 100 predicted to give even map distribution will be used to genotype the international and national reference populations derived from these varieties. The 100 mapped markers will also be run out on acrylamide gels so that polymorphic amplicons can be re-amplified and sequenced to reveal the Avalon and Cadenza SNPs.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	Crop Science, Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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