Award details

Identification, cloning and heterologous expression of the abyssomicin C biosynthetic gene cluster

Reference	BBS/E/J/000CA303
Principal Investigator / Supervisor	Professor Mervyn Bibb
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	1,132
Status	Completed
Type	Institute Project
Start date	02/08/2007
End date	01/08/2010
Duration	36 months

Abstract

Abyssomicin C is a recently discovered antimicrobial natural product that has a completely novel chemical structure, and is the first natural product inhibitor of the essential shikimate pathway present in bacteria and plants. This compound has been synthesized by groups interested in its use as a new pharmaceutical. However, it is unknown how abyssomicin C is biologically synthesized. This is vital both for improving production yields (for potential commercialization), identifying novel enzymes for use in combinatorial biosynthesis, and for the discovery of new compounds with novel activities within this class of natural product. The project will employ a range of techniques including genomic library sequencing and data mining; cloning and heterologous expression; activity screens; and high-density species microarrays to identify both the abyssomicin C biosynthetic gene cluster in Verrucosispora AB-18-032 (producer strain) and homologues in other actinobacterial species. The proposed research will provided a means to exploit this important compound, providing new avenues to tackle the significant problem of antimicrobial drug resistance.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	Microbiology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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