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Investigate the role of RNA structure under the ambient temperature

Reference	BBS/E/J/000C0676
Principal Investigator / Supervisor	Dr Yiliang Ding
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	101,385
Status	Completed
Type	Institute Project
Start date	01/11/2014
End date	31/03/2017
Duration	28 months

Abstract

Plants, as sessile organisms, are continuously exposed to the changes of temperature caused by daily and seasonal fluctuation, called “ambient temperature”. Ambient temperature has an important influence on plant growth, development as well as biotic and abiotic resistance, which eventually significantly influences the yield of crops. Temperature regulated gene expression is highly dependent on alteration of RNA secondary structure, which is very sensitive to temperature change, even as little as one degree. Different species of plants adapt to different ambient temperature (ie: day average temperature (DAT) 16-28 ? for Arabidopsis while 18-32? for rice (Oryza sativa)), indicating global changes of RNA structure from one plant to another. We aim to uncover the global differences of RNA structures of Arabidopsis and rice in response to ambient temperature variation, and the contribution of RNA structure to the difference of ambient temperature for the two model plants. This proposed study aims to understand how plants sense the safe range of ambient temperature between Arabidopsis and rice. We will perform global ribosome profiling and RNA structural profiling under different temperature regimes in rice. We will demonstrate the interplay between ribosome association and RNA structure. We will further determine the differences between the ambient temperature and extreme temperature. With the quantitative measurement of the alteration of RNA structure corresponding to the translation efficiency, we could define the “safe range of temperature” that is finely tuned by RNA structure changes. Furthermore, we will compare these structure features between Arabidopsis and rice to unravel the different effects of ambient temperature.

Summary

unavailable

Committee	Not funded via Committee
Research Topics	Crop Science, Plant Science, Structural Biology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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