Award details

Peptide-mediated signalling in plant disease resistance (SAR peptide signal)

Reference	BBS/E/J/0000A255
Principal Investigator / Supervisor	Professor Chris Lamb
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	56,973
Status	Completed
Type	Institute Project
Start date	01/11/2005
End date	31/08/2007
Duration	22 months

Abstract

Plants use complex recognition and response mechanisms to protect themselves from pathogen attack. Major resistance (R) genes specify recognition of pathogens carrying the corresponding avirulence genes leading to the rapid activation of a battery of inducible defences that is often accompanied by the collapse of challenged plant cells in the hypersensitive response (HR), which results in a restricted lesion clearly delimited from surrounding healthy tissue. In addition, immunity to subsequent attack by a broad range of normally virulent pathogens, so-called systemic acquired resistance (SAR), develops throughout the plant. CDR1 that encode an apoplastic aspartic protease, mediates a peptide signal system involved in the activation of inducible resistance mechanisms. CDR1 over¿expression causes dwarfing and resistance to virulent Pseudomonas syringae. Antisense CDR1 plants were compromised for resistance to avirulent P. syringae and more susceptible to virulent strains than wild type. Little is known about peptide signalling in plants although this mechanism is a regulatory paradigm in animals. Genetics and biochemical dissection of the CDR1-mediated peptide signal in the model plant Arabidopsis should have an impact well beyond the disease resistance area. The aim of this proposal is to determine the nature of the peptide signal generated by CDR1 action. Proteomic techniques or expression of epitope-tagged active site mutants will be used to direct identification of candidate peptides. In addition, phenotypic analysis of CDR1 overexpression and antisense lines crossed with other key signal mutants will help to establish how CDR1 is integrated within the disease resistance signal network and the physiological properties of the peptide signal system. The project will strengthen the experience of the candidate in Plant Molecular Biology, Genetic and Functional Proteomic and will enhance significantly the transfer of knowledge and expertise within the EC.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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