Award details

A highly conserved essential pathway in bacteria

Reference	BBS/E/J/0000A249
Principal Investigator / Supervisor	Professor Tracy Palmer
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	62,110
Status	Completed
Type	Institute Project
Start date	01/10/2005
End date	31/12/2007
Duration	27 months

Abstract

The yjeE gene is highly conserved across all eubacterial species. In E. coli, and other bacteria examined so far, it is essential for vaibility. It encodes a small nucleotide-binding protein that binds ATP with a kD in the region of 80 micromolar, and we propose that it acts as an ATP dependent switch. We have shown that YjeE interacts with another highly conserved essential protein YeaZ and we aim to define the essential cellular process that we propose is regulated by YjeE. The project aims to: [a] Define the network of proteins that interact with YjeE, YeaZ and YgjD (a YeaZ homologue). This will involve a number of approaches including bacterial 2-hybrid studies, immunoprecipitation and affinity purification. Proteins will be purified and interactions studied in vitro using surface plasmon resonance. The effect of nucleotides on complex formation will be assessed. [b] Define the essential pathway in which YjeE, YeaZ and YgjD are involved. This will involve a number of approaches including (i) sub-cellular localisation of YjeE, YeaZ and YgjD using cell fractionation and fluorescence tagging; (ii) transcriptome analysis of cognate depletion strains and (iii) identification of the substrates of YeaZ and YgjD by proteomics and other approaches.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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