Award details

Defining the function and regulation of the SCFHSR5 complex involved in sugar mediated growth control in Arabidopsis

Reference	BBS/E/J/0000A222
Principal Investigator / Supervisor	Professor Michael Bevan
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	236,904
Status	Completed
Type	Institute Project
Start date	28/11/2005
End date	27/11/2008
Duration	36 months

Abstract

All organisms need a supply of nutrients to support growth and development. Sugars such as glucose are universal nutrients as they provide carbon skeletons for energy supply, storage and the synthesis of most metabolites. The central importance of sugar supply to cells is reflected in the complex sensing and signalling systems that have evolved to optimise the appropriate supply of sugars for growth and development. In plants sugars are produced in mature photosynthetic tissues and transported to other regions of the plant for use in long-term storage or for growth. Physiological and biochemical evidence suggests the distribution and allocation of resources is tightly regulated in plants, but so far the cellular mechanisms are relatively poorly understood. We propose to define the function of a novel F box protein in sugar- mediated growth responses in Arabidopsis. This protein, encoded by the High Sugar Response 5 (HSR5) gene, was identified in a screen for Arabidopsis mutants exhibiting elevated expression of sugar- regulated genes and sugar- hypersensitive growth responses. We have established that HSR5 interacts with ASK proteins, which are subunits of the SCF complex that targets proteins for ubiquitination and proteasome- mediated degradation. We will establish if the activity of this SCFHSR5 complex is regulated by glucose and two regulatory proteins previously implicated in sugar responses. We will also identify proteins that may be potential targets for HSR5- mediated degradation and establish their function, in order to establish the cellular processes that may be affected by the SCFHSR5 complex. These analyses will define an important sugar-controlled regulatory network and may also reveal, through a wider definition of the specificity and regulation of proteolysis, a regulatory network involving light, hormones and sugars that integrates growth and development.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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