Award details

A BBSRC contribution to the international IGROW initiative: a protocol for systematically generating wheat mutants and a 1Mb sequenced region in wheat

Reference	BBS/E/J/0000A203
Principal Investigator / Supervisor	Professor Graham Moore
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	155,682
Status	Completed
Type	Institute Project
Start date	01/01/2005
End date	01/06/2007
Duration	29 months

Abstract

The project will make an initial contribution to the International Genome Research On Wheat (IGROW) initiative by generating deletion mutants of the internationally used wheat variety Chinese Spring. We will assess systemically the level of X-Ray dosage required to create specific sizes of deletions in hexaploid wheat. We will use a defined region for which we have PCR-based markers as a means to identify deletions and estimate their size. Six doses of X-Ray will be used to create the mutagenised populations. The average gene speacing in wheat genome is approximately 1 gene every 40Kb. It is predicted that for every 250 genes (a 10MB region) which are deleted in hexaploid wheat, one might give an observable phenotype. We aim to produce deletions which range from 40KB to 10mB in size. Such deletions would encompass between one and 250 genes. IGROW have also asked that any sequences of specific regions are made public to help evaluate the genome analysis approach. My group has produced a contig of 1Mb region in wheat chromosome 5B. We have completed a 2x coverage sequence of the region. The shotgun coverage of this region will be increased to over 6x which from experience provides a high likelihood of uncovering most of the gene content of the region. We will then deposit the annotated sequence and ist comparison with rice and Brachypodium sequences into the public domain.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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