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The role of cyclin dependent protein kinase A in translational control of gene expression in Arabidopsis

Reference	BBS/E/J/0000A201
Principal Investigator / Supervisor	Professor John Doonan
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	99,702
Status	Completed
Type	Institute Project
Start date	16/03/2005
End date	15/03/2008
Duration	36 months

Abstract

Translational control of gene expression is an emerging regulatory mechanism that is implicated in both growth and development. We have found that a key cell cycle regulator, CDKA, and eIF4A are present in the same protein complexes for part of the Arabidopsis cell growth cycle and may couple translation with cell cycle progression or cell growth. We aim to characterise (a) the interaction between the cell cycle regulator, CDKA, and the translational initiation factor, eIF4A and (b) determine how translational activity changes during the cell and growth cycle or in response to CDKA activity. In part A, the specific aims are to characterise the eIF4A-CDKA and other eIF4A complexes and determine if and how they change in protein composition and phosphorylation status during the cell cycle and during growth and cell expansion. In part B, we will examine the effects of down-regulating CDKA activity or eIF4A levels on global and specific translation, as judged by the relative abundance of polyribosome associated mRNAs compared to total mRNAs.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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