Award details

Function of the Exon Junction Complex in the Plant Nucleolus

Reference	BBS/E/J/0000A185
Principal Investigator / Supervisor	Professor Peter Shaw
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	107,050
Status	Completed
Type	Institute Project
Start date	16/10/2004
End date	15/10/2007
Duration	36 months

Abstract

Our recent proteomic analysis of the nucleolus in Arabidopsis has identified a number of interesting and unexpected nucleolar proteins. In particular, we have identified five proteins of the Exon Junction Complex (EJC). The EJC promotes mRNA export via the nuclear pore complex, and couples mRNA export to cytoplasmic nonsense-mediated decay and mRNA localisation. The discovery of the EJC proteins in the plant nucleolus both novel and unexpected. It raises the exciting possibility that this nuclear compartment influences overall expression of the plant proteome by modulating mRNA export, or nuclear mRNA surveillance and nonsense-mediated decay processes. The objectives of the proposal are to use proteomic, molecular, and cell biological analyses to investigate the nature and function of EJC components in the nucleoli of plants. Specific objectives are to determine: 1. the complete protein complement of plant EJCs. 2. the localisation and dynamics of the EJC complex components. 3.the range and nature of mRNAs associated with the nucleolar EJC. 4.whether the nucleolar EJC is involved in nucleolar trafficking or NMD. 5.whether RNAi knockdown of individual components affects the nucleolar EJC, and whether the nucleolar EJC genes are essential.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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