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Functional analysis of DIR1 a putative lipid transfer protein that promotes long distance signalling during systemic acquired resistance in Arabidopsis

Reference	BBS/E/J/0000A179
Principal Investigator / Supervisor	Professor Chris Lamb
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	17,401
Status	Completed
Type	Institute Project
Start date	01/12/2003
End date	30/11/2005
Duration	24 months

Abstract

Attempted infection can result in systemic acquired resistance (SAR)-the development throughout the plant of immunity to subsequent attack by a broad range of normally virulent pathogens. The Arabidopsis mutant defective in induced resistance 1-1 (dirl-1) exhibits wild-type local resistance to avirulent Pseudomonas syringae, but defense gene expression is abolished in distant leaves and dirl-1 fails to develop SAR to virulent Pseudomonas or Peronospora parasitica. Petiole exudates experiments indicate that dirl-1 is defective in the transmission from the inoculated leaf of an essential mobile signal. DIR1 encodes a putative apoplastic lipid transfer protein (LTP) and we propose that DIRl interacts with a lipid-derived molecular to promote long distance signalling. The proposed study will determine how the DIR1 protein functions and thus reveals how to enhance the capacity of plants to develop SAR. The main objectives are proposed to be the following: 1/ localisation of DIR1-LTP and tracking possible relocation of DIR1 during the induction of SAR using DIR1: GFP plants and dexamethasone inducible promoter to monitor the translocation of DIR1. 2/ position of DIR1 relative to other signal components such as EDS1, PAD4 and salicylic acid. This objective will be achieved by using petiole exudates assay and plant genetics. 3/identification and functional characterisation of proteins interacting with DIR1. The main strategy used will be the yeast 2 hybrid screening. This research will underpin genetic delivery of durable, broad spectrum disease resistance in crops and help reduce dependence on agrochemicals in a more sustainable agriculture.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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