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Mechanism of a novel nitric oxide sensor in Escherichia coli

Reference	BBS/E/J/0000A177
Principal Investigator / Supervisor	Professor Raymond Alan Dixon
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	64,309
Status	Completed
Type	Institute Project
Start date	01/01/2004
End date	31/08/2006
Duration	32 months

Abstract

The NorR regulatory protein of Escherichia coli activates expression of a flavorubredoxin (encoded by the norV gene) that is required for resistance to nitric oxide (NO) and reactive nitrogen intermediates (RNIs). NoR activity is only apparent in cells exposed to sources of NO and RNIs, thus NorR functions as a biological sensor of reactive N compounds. Genetic techniques will be used to establish whether there are any other E. coli genes that are required for NorR activity. The NoR protein will be purified and DNA binding and in vitro transcription assays used to identify the signal that is required to induce NorR activity. The signalling domain of NorR will be expressed and purified in isolation for biochemical analysis. A NorR-dependent promoter (from the norV gene) will be characterised, and other targets (if any) for NorR activation will be identified.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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