Award details

Characterization of the EB1 family of microtubule-associated proteins in plants

Reference	BBS/E/J/0000A167
Principal Investigator / Supervisor	Professor Clive Lloyd
Co-Investigators / Co-Supervisors	Professor John Doonan
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	71,414
Status	Completed
Type	Institute Project
Start date	03/11/2003
End date	02/11/2006
Duration	36 months

Abstract

Plant cells do not have microtubule organizing centres. Without markers for MT nucleation we do not know how MT arrays are built, especially the cortical array essential for cell elongation. Promising pilot studies show that GFP-EB1 labels bodies at spindle poles (classical location for MTOCs) and punctae on cortical MTs. Next we propose to clone the small multigene family. Labelling EB1 on plasma membrane disks with immunogold will allow us to visualize the MT nucleation sites and establish the orientation of cortical MTs. EB1 is known to form a complex and we will identify binding partners by affinity tags and proteomics. The developmental role of EB1 will be assayed by RNAi and by screening insertional mutant lines.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search