BBSRC Portfolio Analyser
Award details
Genes and proteins involved in the growth of Rhizobium leguminosarum in biofilms on solid surfaces and legume roots
Reference
BBS/E/J/0000A166
Principal Investigator / Supervisor
Professor J Downie
Co-Investigators /
Co-Supervisors
Institution
John Innes Centre
Department
John Innes Centre Department
Funding type
Research
Value (£)
79,081
Status
Completed
Type
Institute Project
Start date
08/03/2004
End date
07/03/2007
Duration
36 months
Abstract
Rhizobium leguminosarum quorum-sensing mutants are enhanced for biofilm production whereas a protein secretion mutant is defective for biofilm formation. This proposal will use the R. leguminosarum genome sequence to identify candidate genes predicted to be involved in bacterial attachment and growth on surfaces. Targeted and random mutagenesis will identify mutants defective for, and enhanced for biofilm formation. The expression pattern of the identified genes will be analysed in situ on inert surfaces and on legume roots using promoter-gfp fusions. Genes and proteins induced specifically in biofilms will be identified. Analysis of mutant phenotypes on inert surfaces and legume roots will determine the factors required for root attached growth.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search